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Bacteria of fecal origin in mangrove oysters (Crassostrea rhizophorae) in the Cocó River estuary, Ceará State, Brazil BJM
Silva,Ana I.M.; Vieira,Regine H.S.F.; Menezes,Francisca G.R.; Fonteles-Filho,Antonio A.; Torres,Regina C.O.; Sant'Anna,Ernani S..
This study was aimed at evaluating the microbiological quality of mangrove oysters (Crassostrea rhizophorae), collected at a natural oyster bed in the estuary of Cocó river (Fortaleza, Ceará, Brazil). MPN values were used for estimating the total (TC) and fecal (FC) coliforms and Enterococcus spp. TC and FC MPN values in the whole muscle and intervalve liquid ranged from <1.8 to >1,600/g and from <1.8 to 920/g, respectively. The MPN estimates for Enterococcus spp. were between <3.0 and >1,100/g. No correlation was found between the physico-chemical parameters (temperature, salinity and pH) of the surrounding water and the bacteriological contamination levels found in the tested oysters. The only correlation found was between TC and FC...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Oyster; Total and fecal coliforms; Enterococci; Bacteriocins.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000100021
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Bacteriocinogenic effect of Lactobacillus sakei 2a on microbiological quality of fermented Sardinella brasiliensis BABT
Espírito Santo,Milton L. P.; Beirão,Luiz H.; Sant'Anna,Ernani S.; Dalcin,Eliana Bressa; Franco,Bernadette G. M..
Lactobacillus sakei 2a is a bacteriocin producer strain and, in this work, it's effects as a starter culture in the fermentation process of sardine (Sardinella brasiliensis) fillets were observed at different concentrations of NaCl (2, 4 and 6%) and glucose (2 and 4%), to determine it's ability to produce organic acids and consequent pH reduction. Experiments were carried out independently, with only one parameter (NaCl or glucose) varying at a time. After 21 days of fermentation the deteriorative bacteria concentration reached 9.7 Log10 CFU. g-1 corresponding to 6% NaCl and 4% glucose. Little differences were observed in lactic acid production when 2 and 4% glucose were added, since total acidity was 1.32 and 1.34% respectively, the experiments with 6%...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Sardinella brasiliensis; Lactobacillus sakei 2a; Fermented fish.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000400009
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Cultivation of Arthrospira (spirulina) platensis in desalinator wastewater and salinated synthetic medium: protein content and amino-acid profile BJM
Volkmann,Harriet; Imianovsky,Ulisses; Oliveira,Jorge L.B.; Sant'Anna,Ernani S..
Arthrospira (Spirulina) platensis was cultivated in laboratory under controlled conditions (30ºC, photoperiod of 12 hours light/dark provided by fluorescent lamps at a light intensity of 140 µmol photons.m-2.s-1 and constant bubbling air) in three different culture media: (1) Paoletti medium (control), (2) Paoletti supplemented with 1 g.L-1 NaCl (salinated water) and (3) Paoletti medium prepared with desalinator wastewater. The effects of these treatments on growth, protein content and amino acid profile were measured. Maximum cell concentrations observed in Paoletti medium, Paoletti supplemented with salinated water or with desalinator wastewater were 2.587, 3.545 and 4.954 g.L-1, respectively. Biomass in medium 3 presented the highest protein content...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Arthrospira platensis; Desalinator wastewater; Aminogram.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000100022
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Influence of bacteriocins produced by Lactobacillus plantarum BN in the shelf-life of refrigerated bovine meat BJM
Fiorentini,Ângela M.; Sant'Anna,Ernani S.; Porto,Anna C.S.; Mazo,Jaciara Z.; Franco,Bernadette D.G.M..
Sugar cane molasses is a cheap by-product of the sugar cane industry. This product was used for growth and production of bacteriocins by Lactobacillus plantarum BN and evaluated for its potential application in the extension of the shelf-life of raw meat. Bovine meat cubes were dipped in the filtered and neutralized supernatant of the fermented broth (Treatment A) and stored at 5ºC. Counts of psychrotrophic and mesophilic aerobic microorganisms, pH determination and total acidity were performed on meat cubes after 0, 3, 6, 9, 12 and 15 days. These determinations were also done in cubes dipped in a 6% lactic acid solution (treatment B) and distilled water (treatment C). After 3 days, the counts of psychrotrophic microorganisms in cubes submitted to...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Lactobacillus plantarum BN; Bacteriocins; Bovine meat; Shelf-life.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000100010
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Produção de Lactobacillus plantarum em melaço de cana-de-açúcar BABT
Feltrin,Valdemar P.; Sant'Anna,Ernani S.; Porto,Anna C. S.; Torres,Regina C. O..
Comparative studies of Lactobacillus plantarum were carried out in MRS broth (control) and in 3% (w/v) sugar cane blackstrap molasses enriched as follows: 0.5% yeast extract, 0.5% ammonium citrate and 0.5% sodium acetate (Medium 1) and 0.5% yeast extract, 0.5% ammonium citrate, 0.5% sodium acetate, 0.5% ammonium phosphate, 0.2% ammonium citrate, 0.1% Tween 80 and 0.005% manganese sulphate (Medium 2). The experiment, was carried out at a 5 L fermentor, with 3.5 L as working volume. At the end of the fermentation period MRS presented the highest viable cells production (28.68 log CFU/mL) while in Medium 2 it was 25.80 and in Medium 1 19.36 log CFU/mL. Biomass productivity was 0.0865 g.L-1.h for MRS (control), 0.0768 and 0.0507 g.L-1.h for Medium 2 and Medium...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Lactobacillus plantarum; Fermentation; Molasses.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000100015
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The growth of Micrococcus varians by utilizing sugar cane blackstrap molasses as substrate Rev. Microbiol.
Miranda,Luís A. S.; Sant'Anna,Ernani S.; Porto,Anna C. S..
Comparative studies on the growth of Micrococcus varians were carried out in BHI culture medium (control) as well as in a culture medium with 2% diluted sugar cane blackstrap molasses, enriched with 0.1% yeast extract. The experiment was conducted with three samples of the experimental and control media in a 5 liter fermentor with working volume of 3.5 liters, continuous agitation (150 rpm), 35 ± 0.1°C temperature, 0.7 L air. l-1 medium. min -1, initial pH 7.0 ± 0.2, 24 hour fermentation period, and approximate inoculum of 6.0 log10 CFU/ml. Samples were collected at 2-hour intervals. Micrococcus varians grew in the two culture media studied, which confirms the experimental medium viability for the growth of this species. The final average concentration of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Micrococcus varians; Fermentation; Molasses.
Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37141999000200007
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Viability of Staphylococcus xylosus isolated from artisanal sausages for application as starter cultures in meat products BJM
Fiorentini,Ângela Maria; Sawitzki,Maristela Cortez; Bertol,Teresinha Marisa; Sant'Anna,Ernani S..
Viability of Staphylococcus xylosus isolated from artisanal sausages for application as starter cultures in meat products Viability of Staphylococcus xylosus strains AD1 and U5isolated from natural fermented sausages was investigated as starter cultures in fermented sausages produced in the South Region of Brazil. The study demonstrated that the Staphylococcus xylosus strains AD1 and U5showed significant growth during fermentation, stability over freeze-dried process, negative reaction for staphylococcal enterotoxins and viability for using as a single-strain culture or associated with lactic acid bacteria for production of fermented sausages.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Staphylococcus xylosus; Starter cultures; Sausages.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000100022
Registros recuperados: 7
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